Today let’s talk about DNA Sequencing! I sent off my starters to be sequenced and am waiting for the results. But, why are we even doing it? The purpose of DNA sequencing is to determine what types of microbes are present in our samples.  This way we have more to compare when discussing our starters with the class. This also teaches us how different additives effect sourdough starters. Before this week’s class, I knew a little about DNA sequencing. I have studied the results of the wheat based sequences before during work, however I have not ran a sequence myself.

Typically, scientists use NGS technology over Sanger when sequencing. This is because NGS can sequence more samples in a shorter amount of time. Part of this is because NGS has a large library. When a code is read, the program can quickly match it to the best fit.

For this class, we will be using Illumina sequencing by synthesis. Based on the video, the sample has markers added to the ends of them. These markers tell the program where to begin. Once attached, the DNA is read and replicated until there are several copies made. These copies are then read and recorded to be named and compared to others in the sequencing library. I feel like I understand the basics overall in the process, however I am not entirely understanding the first step.